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Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. In addition, the permeation rate was higher in pig skin than in human skin. 2013, Article ID 360239, pp. The Hydrodynamic Radii of Macromolecules and Their The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Drug Deliv. 20K groups (n=3, *: p<0.05, **: p<0.01). The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Peptide and protein transdermal drug delivery. Learn More. For data requests, please contact the authors. Doke SK, Dhawale SC. principles of dynamic light scattering This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES ; Veldhuis, T.F.J. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. J. Learn More. ; Prasad, D.H.L. With FITC-dextran 20 K, cumulative On the molecular weight distribution of dextran T-500. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). J. Braz. In fact, short chains do contain minor branching points. Search 2012;87(6):1728. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Dilute solution properties and degree of chain branching government site. Epub 2021 Feb 9. (2012). Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Elias, Hans-Georg (1975). ; Rjumtsev, E.I. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Drug Deliv. However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of All branched polysaccharide types could be described approximately by the hyperbranching theory. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. (2004). (1991). Pharmacol Pharm. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. This situation is mainly the result of using dextans, solvent, additives, etc. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). 2013;3(5):3588605. Schematic illustration of transdermal delivery after the application of the microporation device. Dynamics of a Polymer Solution in a Rigid Matriz. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Carbohydrate Polymers 95 360-365. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Progr. Arond L.H. Figure5 shows the cumulative amount of the FITC-dextran (M.W. The term f/f0 is sometimes denoted as P, Perrin constant. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. Dextran Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. of different origins. Webdextran hydrodynamic radius. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Polysaccharides-based nanoparticles as drug delivery systems. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Int. (2008). These studies were carried out at the Hisko (Gunpo, Korea). Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. dextran 2009;6(4):34354. 2015;32(5):170413. Microporation applications for enhancing drug delivery. 2011;16(2):518. Intrinsic viscosity of bovine serum albumin conformers. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. The human and pig skin permeability of FITC-dextran (M.W. Int J Food Sci Nutr. ; Ghosh, T.K. For Research Use Only. Dilute solution properties and degree of chain branching for dextran. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. 2005;22(4):5505. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Granath, K. A. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Naik A, Kalia YN, Guy RH. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). International Journal of Pharmaceutics, 277, 99-104. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. 10K, and c M.W. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Format), Citation-(EndNote It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Solvent effects on polysaccharide conformation. The unfolding of iRFP713 in a crowded milieu - PeerJ 2003;89(2):31120. 2004;25(9):155967. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Probiotics Antimicrob Proteins. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. High Manufacturing Standards of Molecular Probes Dextrans. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. IV. The micropores of the human cadaver skin were regenerated according to the time. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Nanoparticles in magnetic resonance imaging: from simple to dual Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 J. Braz. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. (2003). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Carbohydr Polym. (2007). Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). Fluorescent dextran ACS Nano. Ed., 9(2), 85-90. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). 2018;1:10915. Dilute Solution. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Masuelli, M. A. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Molecular dynamics simulations on aqueous We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Catiker, E.; Guner, A. European Polymer Journal, 36, 2143-2146. National Library of Medicine Biomaterials, 22, 1891-1898. Increased TEWL values are evidence of micropores formation. 2010;4(1):117. where tr is the column retention time in minutes (2 of 0.9792). Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. He, L.; Niemeyer, B. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. 2009;8(2):1739. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. All authors read and approved the final manuscript. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. dextran 2012;101(8):28619. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. H&E stained images were analyzed using Image J software. Polyelectrolytes as secondary calibration standards for aqueous SEC. Federal government websites often end in .gov or .mil. (1999). The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. 2012;3(1):208. WebDextran 40 Pharmaceutical Quality. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Keywords: The Mark-Houwink. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Careers. Format). par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth A solvent at this temperature is called a theta solvent [53]. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Carbohydrate Polymers 88 373-381. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Charge: Anionic. Harding, Stephen E. (2005). Proc of SPIE. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. (1954). ; Weisz, J. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Treatment of acne scars by fractional bipolar radiofrequency energy. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. (1954). European Biophysical Journal, 36, 85-94. Hydrodynamic radii ( RH) were determined by dynamic light scattering. FITC-dextran solution (600L) was added to the donor chamber. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. American Journal of Food Science and Technology 2013, 1(3), 60-66. ; Patel, T.R. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. GPC Symp. dextran hydrodynamic radius Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Each microporated sample was incubated in the skin culture medium. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Biophysical Journal, 87, 4259-4270. Pharm Res. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Masuelli, Martin Alberto. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Materiaux 1-5. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Principles of Size Exclusion Chromatography SEC (GFC ; Talsma, H.; Hennink, W.E. This site needs JavaScript to work properly. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. J Control Release. 2011;5(2):95132. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Data File Dextran. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Viscotek GmbH, Europe. ; Fran, H.P. Beer, M.U. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte (2003). Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Hydrodynamic Figure4 shows the histological analysis of micropores in human cadaver skin. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Carbohydrate Polymers, 79, 380-390. Chem. Google Scholar. Clipboard, Search History, and several other advanced features are temporarily unavailable. Intrinsic viscosity molecular weight relationship. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Stokes radius This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa.